The BioBench BioEtOH is a newly launched solid-state fermentation solution from Biostream, capable of performing saccharification and fermentation simultaneously in a single device. It allows for precise control of critical fermentation parameters such as temperature, pH, pO₂, agitation speed, and gas flow, making it an ideal choice for solid-state culture fermentation.

Applications

• Suitable for fermentation of highly viscous and semi-solid samples

• Hydrolysis and simultaneous saccharification and fermentation (SSF)

• Highly efficient mixing effect, facilitating rapid reaction progress

• Simple, convenient, and easy operation for solid sample feeding

• Facilitates temperature and process control for simultaneous saccharification and fermentation

11.6" Touchscreen

• Adjustable touchscreen position, allowing users to move it according to their preferences

• Parameters for up to 6 reactors can be displayed intuitively on a single controller

• Can also be controlled directly using the BOS software on a computer

Open System Design

• Feeding: Configurable with 1–5 peristaltic pumps, with options for external integration of additional pumps

• Gas Lines: Allows on-demand addition of MFCs, rotameters, or manual valves

• Powerful expandable functionality

Data Export

• Optional data export, including setpoints and actual values, ensuring data integrity

Agitation Design

• High-torque stirring motor paired with a specially designed agitator ensures ideal mixing even with highly viscous or relatively dry materials

• Spiral agitator for mixing and gradually lifting the fermentation material layer by layer

• Anchor agitator combined with the spiral agitator enables effective wall-scraping

• Bottom agitator for stirring and lifting bottom materials

• Blade-type agitator for mixing and shearing materials

Flow Control

• Flexible gas mixing control with options for mass flow controllers (MFCs), rotameters, or manual valves

Excellent Temperature Control

• Temperature sensors are installed both inside the vessel and in the jacket water flow, effectively preventing overheating in the center of the vessel and ensuring temperature stability and enzyme activity (essential for enzyme-mediated degradation during fermentation) are not compromised.

Vessel Parameters

• Volume Specifications: 3.6L/2.3L, 5.0L/3.4L

• Integrated thick-walled borosilicate glass cylindrical jacketed vessel with a hemispherical bottom and a 316L stainless steel vessel lid

• Designed for off-site sterilization, easy to disassemble and install; includes a sterilization loop inoculation kit for convenient, safe operation, significantly reducing contamination risks

• Stirring System: Top-mounted agitation with speed control range of 10–230 rpm, accuracy ±1 rpm

• Online Monitoring Probes Provided: Temperature, pH, pO₂, and antifoam probes

• Temperature Control Range: Cooling water temperature +5°C to +65°C, control accuracy ±0.1°C. Culture temperature is controlled via a water jacket covering no less than 70% of the vessel surface area, with continuous forced closed-loop circulation via an internal circulation pump during operation

• pH Detection Range: 2.00–12.00 pH, PID automatic control, accuracy ≤ ±0.01, capable of withstanding high-temperature and high-pressure sterilization

• pO₂ Detection Range: 0%–100%, PID automatic control, accuracy ≤ ±0.1%, capable of withstanding high-temperature and high-pressure sterilization

Control System
The BioBench controller features a touchscreen design that receives signals from various sensors, including temperature, agitation, pH, pO₂, Redox, liquid level, gas flow, pressure control, biomass, current, and voltage standard signals. It offers 30 analog and 22 digital input channels, 10 × Modbus connections for linking drives and control expansion units, and can also connect to monitoring software via the OPC protocol.

Data Logging & Process Control

• Each control loop is independent and modular in design, allowing for flexible operation

• Users can modify parameters based on application requirements to fine-tune control strategies

• Each parameter can be independently monitored (measurement range, calibration), controlled (setpoints, alarm limits, rule adjustment, signal output, PID control data, digital and analog signal outputs), and configured for remote control